我们希望合成荧光染料Lucifer yellow和D-alanine的偶联物（YADA），详见图片。相关的合成路线均有文献支持：

Lucifer yellow anhydride (100 mg,
0.22 mmol), Fmoc‐d‐Dap (215 mg, 0.66 mmol, 3 eq.), and
lithium acetate buffer (20 mL, 1M, pH 5) were added to a 100 mL round bottle.
This solution was stirred at refluxed for 6 h, followed by the addition of an
aqueous solution of potassium (10 mL, 3.35 M) of to form a precipitate. The
precipitate was filtered, washed two times with cold ethanol, and allowed to
dry. The powder was then dissolved in 5 mL of DMF followed by the addition of
DBU (69 μL, 0.46 mmol, 2.1 eq) and stirred at room temperature for 1 h. The
solvent was removed in vacuo and the product was purified via reverse‐phase HPLC (0–90% acetonitrile over
30 min, RT~7 min) The pure fractions were lyophilized to yield the desired
product as a yellow solid as a TFA salt (70.3 mg, 56% yield).

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合成的速度和质量是我们的首要考虑目标，价格可以进一步面议